Answer: The connection of alien DNA is performed at existing restriction sites in one of two antibiotic resistance genes. When the exogenous DNA was ligated into the Sal I site of the tetracycline resistance gene in the vector pBR322, the recombinant plasmid would lose the tetracycline resistance due to the insertion of the exogenous DNA.

How does alien DNA enter plant cells by gene gun?

In the biome approach, foreign DNA enters plant cells by High-speed bombardment of gold or tungsten particlesIn this technique, DNA-coated particles are propelled by a pressurized gun called a gene gun to introduce foreign DNA into plant cells.

Recombination when foreign DNA is linked to a tetracycline resistance gene?

The recombinant lost tetracycline resistance when the foreign gene was ligated into the Sal I site of the tetracycline resistance gene in the vector pBR322 Insert foreign DNA.

What happens if the gene of interest is inserted into the BamHI restriction site of the cloning vector pBR322?

When adding an insert at the BamHI recognition site Tetracycline resistance gene lost function, plasmid pBR322 recombinant bacteria Cells with DNA inserted in BamHI lose tetracycline resistance.

What do you think will happen when a foreign gene is ligated into the PVUI site of the E. coli plasmid pBR322?

When the foreign gene is ligated into the Pvu I site of the ampicillin resistance gene in the vector pBR322 The recombinant plasmid lost ampicillin resistance due to insertion of foreign DNA.

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29 related questions found

Who discovered the plasmid pBR322?

pBR322 is a plasmid and one of the first widely used E. coli cloning vectors.It was created in Herbert Boyer’s laboratory at the University of California, San Francisco in 1977 to Francisco Bolivar ZapataPostdoctoral Fellow and Raymond L. Rodriguez.

How is pBR322 used as a cloning vector?

The pBR322 plasmid contains a gene that enables bacteria to develop resistance to the antibiotics tetracycline and ampicillin. To clone genes using the pBR322 plasmid, Restriction enzymes first cut the plasmid at the restriction site… Another plasmid used as a vector for cloning DNA is called the pUC18 plasmid.

Which produces a blunt end?

When a blunt end is produced Endonuclease cleavage is placed somewhere in the center of the sequence. Sticky ends are created when restriction enzymes cut at the terminal sites, providing loose bonds. Option A: Xho 1: It is isolated from Xanthomonas campestris.

Will any vector be inactivated when foreign DNA is inserted into any vector?

When foreign DNA is inserted into any vector, it causes any marker gene. This is used to select recombinant colonies. Insertional inactivation is an r-DNA technique in which DNA fragments are inserted into restriction sites to inactivate genes.

What type of enzyme is used to cut DNA?

Restriction endonucleases, also known as restriction endonucleases, a protein produced by bacteria that cuts DNA at specific sites in the molecule. In bacterial cells, restriction enzymes cleave foreign DNA, thereby eliminating the infecting organism.

What happens if an Rdna gene with AmpR is transferred into E. coli?

If recombinant DNA with the antibiotic ampicillin resistance gene is transferred into E. coli cells, Transformation of host cells into ampicillin-resistant cells.

Which method is suitable for transferring foreign DNA into plant cells?

gene gun or gene gun Suitable for transferring foreign DNA into plant cells.

How did alien DNA get in?

Foreign DNA can be introduced biolistic host cell. Plant cells are bombarded with DNA-coated gold or tungsten at high speed by this Biolistics or gene gun approach.

Which foreign DNA is inserted into any vector?

Full answer: recombinant DNA technology is a process of joining together DNA from two different species. The recombinant DNA is then administered to the host to develop new combinations. This process is done in three steps, in which the DNA is cut, ligated, and then inserted with the help of a vector.

When foreign DNA is introduced into an organism?

When foreign DNA is introduced into an organism; it is called GMOs or GMOs. It is inserted into the recipient organism with the help of a vector. Then, due to recombination, it becomes an integral part of the host genome like other native genes.

What is it called when a foreign gene is inserted into an organism?

genetically modified or genetically modifiedAn organism is an organism that has been altered through recombinant DNA technology, which involves combining DNA from different genomes or inserting foreign DNA into the genome.

Why are sticky ends better than blunt ends?

because of sticky ends find each other faster due to mutual attraction, the ligation process requires less human DNA and less plasmid DNA. Blunt ends of DNA and plasmids are less likely to find each other, so blunt-end ligation requires more DNA to be put into the tube.

What is the difference between blunt and sticky ends?

Sticky ends get their name because they overlap, allowing the ends to base pair and join together with another DNA strand. blunt ends do not overlap.

Does EcoRI leave blunt or sticky ends?

Created by EcoRI 4 nucleotide sticky ends with 5′ end Overhang of AATT. … other restriction enzymes, depending on their cleavage site, can also leave 3′ overhangs or blunt ends without overhangs.

What does pBR322 stand for?

pBR322 is a plasmid and one of the first widely used E. coli cloning vectors. p stands for « plasmid », BR stands for « bolívar » and « Rodriguez, the scientist who synthesized plasmids. So, the correct answer is ‘Bolivar and Rodriguez

Is pBR322 a cloning vector?

pBR322 DNA is a Commonly used plasmid cloning vectors in E. coli (1). The molecule is a double-stranded loop with a length of 4,361* base pairs (2). pBR322 contains ampicillin and tetracycline resistance genes that can be amplified with chloramphenicol.

What does the 322 in pBR322 mean?

Represented in pBR322,322 Number assigned to isolate it from other types plasmid. or. It stands for synthetic order.

Is pBR322 man-made?

pBR322 is The first artificial cloning vector to be constructed.

Who discovered the plasmid?

The term « plasmid » originated from Joshua Lederberg 1952. He used it to describe « any extrachromosomal genetic factor. » Lederberg first used the term in a paper he published describing some of the experiments he and his graduate student Norton Zinder conducted with Salmonella and its virus, P22.