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Can’t change aperture in Canon manual mode?
00 should be the minimum value and 1/1000 or more is better.
What is the relationship between aperture shutter speed and ISO?
Aperture, Shutter Speed and ISO Combined to control the brightness or darkness of the image (exposure). The same exposure can be achieved using different combinations of aperture, shutter speed and ISO. A larger aperture allows more light to hit the sensor, so the shutter speed can be increased to compensate.